NYMC Faculty Publications

Title

Simultaneous Determination of Tricarboxylic Acid Cycle Metabolites by High-Performance Liquid Chromatography with Ultraviolet Detection

Document Type

Article

Publication Date

6-15-2016

Department

Biochemistry and Molecular Biology

Abstract

Here we describe a simple high-performance liquid chromatography (HPLC) procedure for the simultaneous detection and quantitation in standard solutions of 13 important metabolites of cellular energy metabolism, including 9 tricarboxylic acid (TCA) cycle components and 4 additional metabolites. The metabolites are detected by their absorbance at 210 nm. The procedure does not require prior derivatization, and an analysis can be carried out at ambient temperature within 15 min. The significance of the current work is that the current HPLC procedure should motivate the development of simplified TCA cycle enzyme assays, isotopomer analysis, and determination of selected TCA metabolite levels in plasma/tissues.

Publisher's Statement

The Version of Record can be found in Analytical Biochemistry. https://doi.org/10.1016/j.ab.2016.03.004