Increased Epoxyeicosatrienoic Acids may be Part of a Protective Mechanism in Human Ulcerative Colitis, with Increased CYP2J2 and Reduced soluble Epoxide Hydrolase Expression
BACKGROUND: Previous preclinical evidence has suggested that the elevation of epoxyeicosatrienoic acids (EETs) derived from the cytochrome P450 (CYP) epoxygenases-dependent metabolism of arachidonic acid has important anti-inflammatory effects. However, the levels of EETs and their synthetic and metabolic enzymes in human ulcerative colitis has not been evaluated. METHOD: To evaluate EETs and the expression of relevant CYP isoforms and the metabolizing enzyme, soluble epoxide hydrolase (sEH), tissue biopsies were collected from 16 pairs of ulcerative colitis patients' tissues and matched with adjacent non-inflamed tissues. EETs were extracted from tissue homogenates and analyzed by liquid chromatography coupled with tandem mass spectrometry. RESULTS: The concentration of EETs was higher in ulcerative colitis tissues compared with matched adjacent non-inflamed tissues (1.91+/-0.98ng/mg vs. 0.96+/-0.77ng/mg, mean+/-SD, P0.05). CONCLUSION: Our data suggest that the increase in EET levels may be part of a protective mechanism in ulcerative colitis. Furthermore, the concentration of EETs could be a key factor for drug therapy for ulcerative colitis.
Qiu, Y., Qin, J., Luo, Y., Qin, S., Mu, Y., Cun, R., Jiang, H., Chen, J., Yu, M., & Zhong, M. (2018). Increased Epoxyeicosatrienoic Acids may be Part of a Protective Mechanism in Human Ulcerative Colitis, with Increased CYP2J2 and Reduced soluble Epoxide Hydrolase Expression. Prostaglandins & Other Lipid Mediators, 136, 9-14. https://doi.org/10.1016/j.prostaglandins.2018.03.004