NYMC Faculty Publications

Title

Mechanism of Formation of Non-immune Rosettes Between Guinea-Pig Thymus-Derived Lymphocytes and Rabbit Erythrocytes

First Page

25

Last Page

31

Document Type

Article

Publication Date

January 1977

Department

Pathology

Abstract

Several observations reported here suggest that spontaneous rosette formation between rabbit erythrocytes and guinea-pig T lymphocytes is mediated by natural anti-guinea-pig T-cell antibodies bound to the surface of the rabbit erythrocyte. First, normal rabbit sera frequently contain antibodies specifically cytotoxic for guinea-pig T lymphocytes. Second, the activity of rabbit erythrocytes in spontaneous rosette formation is reduced after incubation for 5 days at pH 6-1, but can be restored to levels seen with fresh erythrocytes by a brief incubation in normal rabbit serum containing natural anti-guinea-pig antibodies; normal serum absorbed with thymocytes does not restore activity to the erythrocytes. Third, the activity of rabbit erythrocytes in forming spontaneous rosettes can be specifically blocked by treatment with anti-allotype and heterologous anti-Ig sera.

This document is currently not available here.

Share

COinS