NYMC Faculty Publications

Title

The Use of 1-Fluoro-2,4-Dinitrobenzene as an Affinity Label for the Antigen Receptor of Delayed Hypersensitivity

Document Type

Article

Publication Date

October 1976

Department

Pathology

Abstract

The passive transfer of delayed contact sensitivity to dinitrophenyl can be specifically inhibited by brief treatment of lymph node cells (LNC) from sensitized guinea-pigs with 1-fluoro-2,4-dinitrobenzene (DNFB). Analysis of KC1 extracts of LNC previously treated with (3H)DNFB, using polyacrylamide gel electrophoresis, revealed a peak of radioactivity present in both sensitive and normal cell extracts as well as a peak found only in sensitive cell extracts. Cell-free extracts appeared capable of transmitting delayed-onset skin reactivity to non-sensitized recipients.

This document is currently not available here.

Share

COinS