NYMC Faculty Publications
Receptor Activator of Nuclear Factor Kappa B Ligand Antagonists Inhibit Tissue Inflammation and Bone Loss in Experimental Periodontitis
Author Type(s)
Faculty
Additional Author Affiliation
Touro College of Dental Medicine at NYMC
DOI
10.1111/j.1600-051X.2011.01780.x
Journal Title
Journal of Clinical Periodontology
First Page
1029
Last Page
1036
Document Type
Article
Publication Date
11-1-2011
Department
Pharmacology
Keywords
Alveolar Bone Loss, Animals, Chemokine CCL2, Interleukin-6, Male, Mice, Mice, Inbred C57BL, Osteoprotegerin, Periodontitis, Porphyromonas gingivalis, Pyrones, RANK Ligand, Random Allocation, Recombinant Fusion Proteins, Tumor Necrosis Factor-alpha
Disciplines
Medicine and Health Sciences
Abstract
AIM: The purpose of this study was to assess the role of anti-bone resorptive agents and an anti-inflammatory compound in murine Porphyromonas gingivalis (P. gingivalis)-induced periodontitis.
MATERIAL AND METHODS: Six randomly assigned groups were administered vehicle (saline, control) (n = 6), P. gingivalis infection only (untreated) (n = 6), human-Fc (n = 4), Kavain (n = 6), OPG-Fc (n = 6) and Receptor activator of nuclear factor-kappa B (RANK)-Fc (n = 6) intraperitoneally at day 0, 3 and 7. Animals were euthanized on day 10 and subjected to comprehensive histomorphometric analysis. To capture the progress of inflammation, serum samples were collected at days 0, 3, 7 and 10 for levels of pro-inflammatory cytokines.
RESULTS: Compared with control group, OPG-Fc, RANK-Fc and Kavain treatment showed significant bone loss reduction with OPG-Fc performing better than RANK-Fc or Kavain. Epithelial down-growth showed significant reduction in treatment groups with OPG-Fc performing better than RANK-Fc or Kavain. Finally, Kavain, OPG-Fc and RANK-Fc-treated mice displayed reduced inflammatory cell counts and cytokine expression particularly at day 7 postinfection.
CONCLUSIONS: RANKL antagonists and Kavain effectively reduced alveolar bone loss in P. gingivalis-induced periodontitis in our mice model. Compared with RANK-Fc, Kavain-treated animals showed milder improvement of bone and connective tissue inflammation. Therapeutic implications in the prevention of periodontal bone loss are discussed.
Recommended Citation
Yuan, H., Gupte, R., Zelkha, S., & Amar, S. (2011). Receptor Activator of Nuclear Factor Kappa B Ligand Antagonists Inhibit Tissue Inflammation and Bone Loss in Experimental Periodontitis. Journal of Clinical Periodontology, 38 (11), 1029-1036. https://doi.org/10.1111/j.1600-051X.2011.01780.x
