NYMC Faculty Publications
Col-F, a Fluorescent Probe for Ex Vivo Confocal Imaging of Collagen and Elastin in Animal Tissues
Author Type(s)
Faculty
DOI
10.1002/cyto.a.22264
Journal Title
Cytometry. Part A : the Journal of the International Society for Analytical Cytology
First Page
533
Last Page
539
Document Type
Article
Publication Date
6-1-2013
Department
Pathology, Microbiology and Immunology
Abstract
A new low-molecular-weight fluorescent probe, Col-F, that exhibits affinity to collagen and elastin, was used successfully in imaging of extracellular matrix in freshly excised animal tissues. Col-F readily penetrates between live cells into tissues and binds to fibers of collagen and elastin by a noncovalent mechanism. Fibers of collagen and elastin have been stained in a variety of tissues, including tendon, skeletal muscle, connective tissue, and arteries. Cells migrating in a Col-F-stained collagenous biomaterial were also imaged. No phototoxic effects were detected when live keratocytes were imaged in the in vitro culture in the presence of Col-F. In conclusion, Col-F provides a simple and convenient tool for fluorescence three-dimensional imaging of intricate collagenous and elastic structures in live and fixed animal tissues, as well as in collagen-containing biomaterials.
Recommended Citation
Biela, E., Galas, J., Lee, B., Johnson, G., Darzynkiewicz, Z., & Dobrucki, J. (2013). Col-F, a Fluorescent Probe for Ex Vivo Confocal Imaging of Collagen and Elastin in Animal Tissues. Cytometry. Part A : the Journal of the International Society for Analytical Cytology, 83 (6), 533-539. https://doi.org/10.1002/cyto.a.22264
