Ocular Hypertension/Glaucoma in Minipigs: Episcleral Veins Cauterization and Microbead Occlusion Methods

Authors

Elena Vecino
Haritz Urcola
Alejando Bayon
Sansar C. Sharma, New York Medical CollegeFollow

DOI

10.1007/978-1-4939-7407-8_4

Journal Title

Methods in Molecular Biology

First Page

41

Last Page

48

Document Type

Article

Publication Date

1-1-2018

Department

Ophthalmology

Abstract

Two methods to induce elevation of the intraocular pressure (experimental glaucoma) are described in the present chapter. The first method is based on increasing the post-trabecular resistance to aqueous outflow by cauterizing the episcleral veins (EVC). This method allows the observation of ultrastructural changes in the trabecular meshwork (TM) without interfering with any structure within the eye such as TM, ciliary body, and/or the Retina. The second method is the multiple injection of microbeads into the anterior chamber, as a pre and intra-trabecular method that induce secondary effects on the TM cells. Both methods lead to an increase in IOP.

Recommended Citation

Vecino, E., Urcola, H., Bayon, A., & Sharma, S. (2018). Ocular Hypertension/Glaucoma in Minipigs: Episcleral Veins Cauterization and Microbead Occlusion Methods. Methods in Molecular Biology, 1695, 41-48. https://doi.org/10.1007/978-1-4939-7407-8_4