NYMC Faculty Publications
DOI
10.1161/ATVBAHA.118.311171
Journal Title
Arteriosclerosis, Thrombosis, and Vascular Biology
First Page
2184
Last Page
2190
Document Type
Article
Publication Date
9-1-2018
Department
Pharmacology
Abstract
OBJECTIVE: Unreliable antibodies often hinder the accurate detection of an endogenous protein, and this is particularly true for the cardiac and smooth muscle cofactor, MYOCD (myocardin). Accordingly, the mouse Myocd locus was targeted with 2 independent epitope tags for the unambiguous expression, localization, and activity of MYOCD protein. APPROACH AND RESULTS: 3cCRISPR (3-component clustered regularly interspaced short palindromic repeat) was used to engineer a carboxyl-terminal 3xFLAG or 3xHA epitope tag in mouse embryos. Western blotting with antibodies to each tag revealed a protein product of approximately 150 kDa, a size considerably larger than that reported in virtually all publications. MYOCD protein was most abundant in some adult smooth muscle-containing tissues with surprisingly low-level expression in the heart. Both alleles of Myocd are active in aorta because a 2-fold increase in protein was seen in mice homozygous versus heterozygous for FLAG-tagged Myocd. ChIP-quantitative polymerase chain reaction studies provide proof-of-principle data demonstrating the utility of this mouse line in conducting genome-wide ChIP-seq studies to ascertain the full complement of MYOCD-dependent target genes in vivo. Although FLAG-tagged MYOCD protein was undetectable in sections of adult mouse tissues, low-passaged vascular smooth muscle cells exhibited expected nuclear localization. CONCLUSIONS: This report validates new mouse models for analyzing MYOCD protein expression, localization, and binding activity in vivo and highlights the need for rigorous authentication of antibodies in biomedical research.
Recommended Citation
Lyu, Q., Dhagia, V., Han, Y., Guo, B., Wines-Samuelson, M., Christie, C., Yin, Q., Slivano, O., Herring, P., Long, X., Gupte, S., & Miano, J. (2018). CRISPR-Cas9 Mediated Epitope Tagging Provides Accurate and Versatile Assessment of Myocardin--Brief Report. Arteriosclerosis, Thrombosis, and Vascular Biology, 38 (9), 2184-2190. https://doi.org/10.1161/ATVBAHA.118.311171
Publisher's Statement
This is the author's accepted manuscript. The final publisher version is available at https://doi.org/10.1161/ATVBAHA.118.311171