NYMC Faculty Publications
Cloning and DNA Sequence Analysis of bmpC, a Gene Encoding a Potential Membrane Lipoprotein of Borrelia Burgdorferi
DOI
10.1111/j.1574-6968.1994.tb07204.x
Journal Title
FEMS Microbiology Letters
First Page
75
Last Page
82
Document Type
Article
Publication Date
October 1994
Department
Pathology, Microbiology and Immunology
Keywords
Amino Acid Sequence; Bacterial Outer Membrane Proteins/genetics; Base Sequence; Borrelia burgdorferi Group/genetics; Cloning, Molecular; Escherichia coli/genetics; Genes, Bacterial; Genomic Library; Molecular Sequence Data; Sequence Alignment; Treponema pallidum/genetics
Disciplines
Medicine and Health Sciences
Abstract
Immunoscreening of a lambda gt11 genomic library of Borrelia burgdorferi expressed in Escherichia coli permitted detection of a clone containing a partial sequence of a B. burgdorferi gene encoding a protein with significant homology to TmpC of Treponema pallidum. Subsequent cloning and DNA sequence analysis revealed an open reading frame encoding a protein with 353 amino acid residues. The open reading frame is preceded by putative promoter sequences and a ribosome binding site, and is initiated with a TTG. The putative protein shares 26% identity with TmpC, contains a signal peptidase II sequence, and is also homologous to the gene products of the recently described bmpA and bmpB of B. burgdorferi. This gene has been designated bmpC. Additional sequencing and restriction analysis indicate that it is located at approximately 400 kbp on the chromosomal map of B. burgdorferi, immediately upstream of bmpA and bmpB.
Recommended Citation
Aron, L., Alekshun, M., Perlee, L., Schwartz, I., Godfrey, H., & Cabello, F. (1994). Cloning and DNA Sequence Analysis of bmpC, a Gene Encoding a Potential Membrane Lipoprotein of Borrelia Burgdorferi. FEMS Microbiology Letters, 123 (1-2), 75-82. https://doi.org/10.1111/j.1574-6968.1994.tb07204.x
