NYMC Faculty Publications

DOI

10.1111/j.1574-6968.2010.02020.x

Journal Title

FEMS Microbiology Letters

First Page

77

Last Page

83

Document Type

Article

Publication Date

8-1-2010

Department

Pathology, Microbiology and Immunology

Abstract

BmpA is an immunodominant protein of Borrelia burgdorferi as well as an arthritogenic factor. Rabbit antirecombinant BmpA (rBmpA) antibodies were raised, characterized by assaying their cross reactivity with rBmpB, rBmpC and rBmpD, and then rendered monospecific by absorption with rBmpB. This monospecific reagent reacted only with rBmpA in dot immunobinding and detected a single 39 kDa, pI 5.0, spot on two-dimensional immunoblots. It was used to assess the BmpA cellular location. BmpA was present in both detergent-soluble and -insoluble fractions of Triton X-114 phase-partitioned borrelial cells, suggesting that it was a membrane lipoprotein. Immunoblots of proteinase K-treated intact and Triton X-100 permeabilized cells showed digestion of BmpA in intact cells, consistent with surface exposure. This exposure was confirmed by dual-label immunofluorescence microscopy of intact and permeabilized borrelial cells. Conservation and surface localization of BmpA in all B. burgdorferi sensu lato genospecies could point to its playing a key role in this organism's biology and pathobiology.

Publisher's Statement

This is the accepted manuscript version of this article. The publisher's final edited version of this article is available at https://doi.org/10.1111/j.1574-6968.2010.02020.x

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