NYMC Faculty Publications
Isoelectrophoretic Characterization of Protein Antigens Present in Mycobacterial Culture Filtrates and Recognized by Monoclonal Antibodies Directed Against the Mycobacterium Bovis Bcg Antigen 85 Complex
DOI
10.1111/j.1365-3083.1992.tb03130.x
Journal Title
Scandinavian Journal of Immunology
First Page
697
Last Page
702
Document Type
Article
Publication Date
November 1992
Department
Pathology, Microbiology and Immunology
Abstract
Nine monoclonal antibodies (MoAbs) were produced against Mycobacterium bovis BCG antigen 85 complex. Using isoelectric focusing combined with Western (immunoblot) blot analysis, antigenically related proteins could be identified in culture filtrates from M. tuberculosis, M. bovis, M. Kansasii, M. avium, M. xenopi, M. gordonae, M. fortuitum, M. phlei and M. smegmatis. Most of the MoAbs were found to be broadly cross-reactive between the various mycobacterial species, albeit some minor differences were observed. These MoAbs reacted generally, in each species, with different components. One MoAb (VID1-14) was found to be specifically directed only against antigen 85B from M. bovis, M. tuberculosis and M. kansasii.
Recommended Citation
Drowart, A., De Bruyn, J., Huygen, K., Damiani, G., Godfrey, H., Stelandre, M., Yernault, J., & Van Vooren, J. (1992). Isoelectrophoretic Characterization of Protein Antigens Present in Mycobacterial Culture Filtrates and Recognized by Monoclonal Antibodies Directed Against the Mycobacterium Bovis Bcg Antigen 85 Complex. Scandinavian Journal of Immunology, 36 (5), 697-702. https://doi.org/10.1111/j.1365-3083.1992.tb03130.x
