NYMC Faculty Publications
Antibodies Produced After Immunization With Substituted Meta-Dinitrobenzenes. Production of Gamma 1 and Gamma 2 Classes of Antibody Including Complement-Dependent Antibodies
Journal Title
Journal of Immunology (Baltimore, Md.: 1950)
First Page
1201
Last Page
1212
Document Type
Article
Publication Date
May 1971
Department
Pathology, Microbiology and Immunology
Disciplines
Medicine and Health Sciences
Abstract
The serum antibodies induced in guinea pigs after immunization with 5 µmols of a series of substituted meta-dinitrobenzenes in Freund's adjuvant were investigated. The frequency of induction of precipitating and anaphylactic antibodies was directly related to the chemical reactivity of the immunizing material; no such relation was found for induction of hemagglutinating antibodies. The most reactive chemicals and one preformed conjugate (DNP37BSA) induced antibodies in more than 80% of the animals but the types of antibody varied. 2,4-Dinitrofluorobenzene induced high titers of anaphylactic antibodies, high levels of γ1 immunoglobulins and an antibody of γ1 mobility whose precipitation was complement-dependent. 3,5-Dinitrobenzoyl chloride induced high titers of hemagglutinating antibody, high levels of γ2 immunoglobulins and a γ2-precipitating antibody whose precipitation was complement-independent. The conjugate induced equal titers of anaphylactic and hemagglutinating antibodies and equal amounts of γ1 and γ2 precipitins. The antibodies induced were extensively cross-reactive, in contrast to the great specificity shown earlier for the delayed response to these chemicals.
Recommended Citation
Godfrey, H. P., & Baer, H. (1971). Antibodies Produced After Immunization With Substituted Meta-Dinitrobenzenes. Production of Gamma 1 and Gamma 2 Classes of Antibody Including Complement-Dependent Antibodies. Journal of Immunology (Baltimore, Md.: 1950), 106 (5), 1201-1212. Retrieved from https://touroscholar.touro.edu/nymc_fac_pubs/1710
