NYMC Faculty Publications

Title

Antibodies Produced After Immunization With Substituted Meta-Dinitrobenzenes. Production of Gamma 1 and Gamma 2 Classes of Antibody Including Complement-Dependent Antibodies

Document Type

Article

Publication Date

May 1971

Department

Pathology

Abstract

The serum antibodies induced in guinea pigs after immunization with 5 µmols of a series of substituted meta-dinitrobenzenes in Freund's adjuvant were investigated. The frequency of induction of precipitating and anaphylactic antibodies was directly related to the chemical reactivity of the immunizing material; no such relation was found for induction of hemagglutinating antibodies. The most reactive chemicals and one preformed conjugate (DNP37BSA) induced antibodies in more than 80% of the animals but the types of antibody varied. 2,4-Dinitrofluorobenzene induced high titers of anaphylactic antibodies, high levels of γ1 immunoglobulins and an antibody of γ1 mobility whose precipitation was complement-dependent. 3,5-Dinitrobenzoyl chloride induced high titers of hemagglutinating antibody, high levels of γ2 immunoglobulins and a γ2-precipitating antibody whose precipitation was complement-independent. The conjugate induced equal titers of anaphylactic and hemagglutinating antibodies and equal amounts of γ1 and γ2 precipitins. The antibodies induced were extensively cross-reactive, in contrast to the great specificity shown earlier for the delayed response to these chemicals.

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