Nucleolin Is a Functional Binding Protein for Salinomycin in Neuroblastoma Stem Cells

Authors

Fengfei Wang
Shuang Zhou
Dan Qi
Shi-Hua Xiang
Eric T. Wong
Xuejing Wang
Ekokobe Fonkem
Tze-Chen Hsieh, New York Medical CollegeFollow
Jianhua Yang
Batool Kirmani
John B. Shabb
Joseph M. Wu, New York Medical CollegeFollow
Min Wu
Jason H. Huang
Wei-Hsuan Yu
Erxi Wu

DOI

10.1021/jacs.8b12872

Journal Title

Journal of the American Chemical Society

First Page

3613

Last Page

3622

Document Type

Article

Publication Date

February 2019

Department

Biochemistry and Molecular Biology

Abstract

The aim of this study is to illuminate a novel therapeutic approach by identifying a functional binding target of salinomycin, an emerging anticancer stem cell (CSC) agent, and to help dissect the underlying action mechanisms. By utilizing integrated strategies, we identify that nucleolin (NCL) is likely a salinomycin-binding target and a critical regulator involved in human neuroblastoma (NB) CSC activity. Salinomycin markedly suppresses NB CD34 expression and reduces CD34+ cell population in an NCL-dependent manner via disruption of the interaction of NCL with CD34 promoter. The elevated levels of NCL expression in NB tumors are associated with poor patient survival. Altogether, these results indicate that NCL is likely a novel functional salinomycin-binding target that exhibits the potential to be a prognostic marker for NB therapy.

Recommended Citation

Wang, F., Zhou, S., Qi, D., Xiang, S., Wong, E., Wang, X., Fonkem, E., Hsieh, T., Yang, J., Kirmani, B., Shabb, J., Wu, J., Wu, M., Huang, J., Yu, W., & Wu, E. (2019). Nucleolin Is a Functional Binding Protein for Salinomycin in Neuroblastoma Stem Cells. Journal of the American Chemical Society, 141 (8), 3613-3622. https://doi.org/10.1021/jacs.8b12872