NYMC Faculty Publications
CC Chemokine CCL1 receptor CCR8 Mediates Conversion of Mesenchymal Stem Cells to Embryoid Bodies Expressing FOXP3+CCR8+ Regulatory T Cells
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NYMC First Author Publication with the Highest Usage
DOI
10.1371/journal.pone.0218944
Journal Title
PLoS One
First Page
e0218944
Document Type
Article
Publication Date
7-1-2019
Department
Pathology, Microbiology and Immunology
Abstract
Embryoid bodies (EBs) are three dimensional aggregates of pluripotent stem cells primarily used to investigate morphogenesis and cell toxicity, are also attractive tools in regenerative medicine. While embryonic stem cells (ESCs) and induced pluripotent cells (IPSCs) have been shown to form EBs in mouse, primate and humans, EB formation have not been previously demonstrated in mesenchymal stem cells (MSCs). Here we show that rat MSCs form EBs; which express regulatory T cell (Treg) marker Foxp3 and CC chemokine CCL1 receptor CCR8. We show a novel method for formation of EBs from MSCs under stress and demonstrate that the induction of FoxP3+ CCR8+ EBs is dependent upon CCL1 gradients which mediate cell proliferation, migration and invasion of mTregs. The identification of EBs and novel FoxP3+ CCR8+ regulatory T cells (mTregs) for selective conversion and isolation of bone marrow derived MSCs offers novel avenues for research, diagnosis and treatment.
Recommended Citation
Haque, N. S., Tuteja, A., & Haque, N. (2019). CC Chemokine CCL1 receptor CCR8 Mediates Conversion of Mesenchymal Stem Cells to Embryoid Bodies Expressing FOXP3+CCR8+ Regulatory T Cells. PLoS One, 14 (7), e0218944. https://doi.org/10.1371/journal.pone.0218944