40 Years of My Venture with CYTOMETRY

Authors

Zbigniew Darzynkiewicz, New York Medical CollegeFollow

DOI

10.1002/cyto.a.24043

Journal Title

Cytometry. Part A : the journal of the International Society for Analytical Cytology

First Page

557

Last Page

562

Document Type

Editorial

Publication Date

6-2020

Department

Pathology, Microbiology and Immunology

Keywords

Acridine Orange, Cell Cycle, DNA, Flow Cytometry, Fluorescent Dyes

Disciplines

Medicine and Health Sciences | Research Methods in Life Sciences | Teacher Education and Professional Development

Abstract

Briefly depicted are the publications in CYTOMETRY that received the highest frequency of citations. Among them are seminal papers describing application of metachromatic fluorochrome acridine orange to differentially stain DNA versus RNA or to analyze susceptibility of DNA in situ to denaturation; both features being markers of different sections of the cell cycle including identification of noncycling quiescent cells. The papers reviewing detection of cyclins D1, E, A or B1, each in relation to cell cycle phase, were also among the highly cited ones. The highest citation rates received publications describing development of the TUNEL methodology to detect apoptotic DNA fragmentation, and more recently expression of ϒH2AX to reveal DNA damage. © 2020 International Society for Advancement of Cytometry.

Recommended Citation

Darzynkiewicz, Z. (2020). 40 Years of My Venture with CYTOMETRY. Cytometry. Part A : the journal of the International Society for Analytical Cytology, 97 (6), 557-562. https://doi.org/10.1002/cyto.a.24043