NYMC Faculty Publications
Proximal Tubular-Targeted Overexpression of the Cyp4a12-20-HETE Synthase Promotes Salt-Sensitive Hypertension in Male Mice
DOI
10.1152/ajpregu.00089.2020
Journal Title
American Journal of Physiology. Regulatory, Integrative and Comparative Physiology
First Page
87
Last Page
95
Document Type
Article
Publication Date
6-17-2020
Department
Physiology
Second Department
Pharmacology
Abstract
20-Hydroxyeicosatetraenoic acid (20-HETE) has been linked to blood pressure (BP) regulation via actions on the renal microvasculature and tubules. We assessed tubular 20-HETE contribution to hypertension by generating transgenic mice overexpressing the CYP4A12-20-HETE synthase (PT-4a12 mice) under the control of the proximal tubule (PT)-specific promoter, phosphoenolpyruvate carboxykinase (PEPCK). 20-HETE levels in the kidney cortex of male (967±210 vs. 249±69 pg/mg protein), but not female (121±15 vs. 92±11 pg/mg protein) PT-4a12 mice, showed a 2.5-fold increase compared to WT. Renal cortical Cyp4a12 mRNA and CYP4A12 protein in male, but not female PT-4a12 mice increased by 2-3-fold compared to WT. Male PT-4a12 mice displayed elevated BP (142±1 vs. 111±4 mmHg, p
Recommended Citation
Gilani, A., Agostinucci, K., Pascale, J. V., Hossain, S., Kandhi, S., Pandey, V., Garcia, V., Nasjletti, A., & Schwartzman, M. L. (2020). Proximal Tubular-Targeted Overexpression of the Cyp4a12-20-HETE Synthase Promotes Salt-Sensitive Hypertension in Male Mice. American Journal of Physiology. Regulatory, Integrative and Comparative Physiology, 319 (1), 87-95. https://doi.org/10.1152/ajpregu.00089.2020
