NYMC Faculty Publications

Activated Microglia Cause Metabolic Disruptions in Developmental Cortical Interneurons that Persist in Interneurons from Individuals with Schizophrenia

DOI

10.1038/s41593-020-00724-1

Journal Title

Nature Neuroscience

First Page

1352

Last Page

1364

Document Type

Article

Publication Date

11-2020

Department

Pathology, Microbiology and Immunology

Second Department

Biostatistics

Third Department

Cell Biology and Anatomy

Abstract

The mechanisms by which prenatal immune activation increase the risk for neuropsychiatric disorders are unclear. Here, we generated developmental cortical interneurons (cINs)-which are known to be affected in schizophrenia (SCZ) when matured-from induced pluripotent stem cells (iPSCs) derived from healthy controls (HCs) and individuals with SCZ and co-cultured them with or without activated microglia. Co-culture with activated microglia disturbed metabolic pathways, as indicated by unbiased transcriptome analyses, and impaired mitochondrial function, arborization, synapse formation and synaptic GABA release. Deficits in mitochondrial function and arborization were reversed by alpha lipoic acid and acetyl-L-carnitine treatments, which boost mitochondrial function. Notably, activated-microglia-conditioned medium altered metabolism in cINs and iPSCs from HCs but not in iPSCs from individuals with SCZ or in glutamatergic neurons. After removal of activated-microglia-conditioned medium, SCZ cINs but not HC cINs showed prolonged metabolic deficits, which suggests that there is an interaction between SCZ genetic backgrounds and environmental risk factors.

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