NYMC Faculty Publications
Simultaneous Determination of Tricarboxylic Acid Cycle Metabolites by High-Performance Liquid Chromatography with Ultraviolet Detection
First Page
8
Last Page
10
Document Type
Article
Publication Date
6-15-2016
Department
Biochemistry and Molecular Biology
Abstract
Here we describe a simple high-performance liquid chromatography (HPLC) procedure for the simultaneous detection and quantitation in standard solutions of 13 important metabolites of cellular energy metabolism, including 9 tricarboxylic acid (TCA) cycle components and 4 additional metabolites. The metabolites are detected by their absorbance at 210 nm. The procedure does not require prior derivatization, and an analysis can be carried out at ambient temperature within 15 min. The significance of the current work is that the current HPLC procedure should motivate the development of simplified TCA cycle enzyme assays, isotopomer analysis, and determination of selected TCA metabolite levels in plasma/tissues.
Recommended Citation
Shurubor, Y. I., Cooper, A. J., Isakova, E. P., Deryabina, Y. I., Beal, M. F., & Krasnikov, B. F. (2016). Simultaneous determination of tricarboxylic acid cycle metabolites by high-performance liquid chromatography with ultraviolet detection. Analytical Biochemistry, 503, 8-10. doi:10.1016/j.ab.2016.03.004
Publisher's Statement
The Version of Record can be found in Analytical Biochemistry. https://doi.org/10.1016/j.ab.2016.03.004