NYMC Faculty Publications
Using Single-Nucleus RNA-Sequencing to Interrogate Transcriptomic Profiles of Archived Human Pancreatic Islets
Author Type(s)
Faculty
Journal Title
Genome Medicine
First Page
128
Last Page
128
Document Type
Article
Publication Date
8-10-2021
Department
Pharmacology
Abstract
BACKGROUND: Human pancreatic islets are a central focus of research in metabolic studies. Transcriptomics is frequently used to interrogate alterations in cultured human islet cells using single-cell RNA-sequencing (scRNA-seq). We introduce single-nucleus RNA-sequencing (snRNA-seq) as an alternative approach for investigating transplanted human islets.
METHODS: The Nuclei EZ protocol was used to obtain nuclear preparations from fresh and frozen human islet cells. Such preparations were first used to generate snRNA-seq datasets and compared to scRNA-seq output obtained from cells from the same donor. Finally, we employed snRNA-seq to obtain the transcriptomic profile of archived human islets engrafted in immunodeficient animals.
RESULTS: We observed virtually complete concordance in identifying cell types and gene proportions as well as a strong association of global and islet cell type gene signatures between scRNA-seq and snRNA-seq applied to fresh and frozen cultured or transplanted human islet samples.
CONCLUSIONS: We propose snRNA-seq as a reliable strategy to probe transcriptomic profiles of freshly harvested or frozen sources of transplanted human islet cells especially when scRNA-seq is not ideal.
Recommended Citation
Basile, G., Kahraman, S., Dirice, E., Pan, H., Dreyfuss, J. M., & Kulkarni, R. N. (2021). Using Single-Nucleus RNA-Sequencing to Interrogate Transcriptomic Profiles of Archived Human Pancreatic Islets. Genome Medicine, 13 (1), 128-128. https://doi.org/10.1186/s13073-021-00941-8
