NYMC Faculty Publications

Expression in the Adult Mouse Heart Modulates Na Influx in Myocytes and Reveals a Mechanistic Link Between Na Entry and Diastolic Function

Author Type(s)

Faculty

Journal Title

American Journal of Physiology. Heart and Circulatory Physiology

First Page

H975

Last Page

H993

Document Type

Article

Publication Date

6-1-2022

Department

Pediatrics

Second Department

Pathology, Microbiology and Immunology

Third Department

Medicine

Abstract

Voltage-gated sodium channels (VGSCs) are macromolecular assemblies composed of a number of proteins regulating channel conductance and properties. VGSCs generate Na current () in myocytes and play fundamental roles in excitability and impulse conduction in the heart. Moreover, VGSCs condition mechanical properties of the myocardium, a process that appears to involve the late component of . Variants in the gene , encoding the VGSC β1- and β1B-subunits, result in inherited neurological disorders and cardiac arrhythmias. But the precise contributions of β1/β1B-subunits and VGSC integrity to the overall function of the adult heart remain to be clarified. For this purpose, adult mice with cardiac-restricted, inducible deletion of (conditional knockout, cKO) were studied. Myocytes from cKO mice had increased densities of fast (+20%)- and slow (+140%)-inactivating components of , with respect to control cells. By echocardiography and invasive hemodynamics, systolic function was preserved in cKO mice, but diastolic properties and ventricular compliance were compromised, with respect to control animals. Importantly, inhibition of late with GS967 normalized left ventricular filling pattern and isovolumic relaxation time in cKO mice. At the cellular level, cKO myocytes presented delayed kinetics of Ca transients and cell mechanics, defects that were corrected by inhibition of . Collectively, these results document that VGSC β1/β1B-subunits modulate electrical and mechanical function of the heart by regulating, at least in part, Na influx in cardiomyocytes. We have investigated the consequences of deletion of , the gene encoding voltage-gated sodium channel β1-subunits, on myocyte and cardiac function. Our findings support the notion that expression controls properties of Na influx and Ca cycling in cardiomyocytes affecting the modality of cell contraction and relaxation. These effects at the cellular level condition electrical recovery and diastolic function in vivo, substantiating the multifunctional role of β1-subunits in the physiology of the heart.

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