Detection of Antibodies to Anaplasma Phagocytophilum and Babesia Microti Using Linear Peptides

Authors

• Teresa Tagliafierro, Center for Infection and Immunity, Mailman School of Public Health, Columbia University, 722 West 168th Street, Room 1701, New York, NY 10032, United States.
• Shreyas Joshi, Center for Infection and Immunity, Mailman School of Public Health, Columbia University, 722 West 168th Street, Room 1701, New York, NY 10032, United States.
• Stephen Sameroff, Center for Infection and Immunity, Mailman School of Public Health, Columbia University, 722 West 168th Street, Room 1701, New York, NY 10032, United States.
• Adriana Marques, Laboratory of Clinical Immunology and Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, United States.Follow
• J Stephen Dumler, Department of Pathology, Walter Reed National Military Medical Center, Uniformed Services University of the Health Sciences, Bethesda, MD, United States.
• Nischay Mishra, Center for Infection and Immunity, Mailman School of Public Health, Columbia University, 722 West 168th Street, Room 1701, New York, NY 10032, United States; Department of Epidemiology, Mailman School of Public Health, Columbia University, New York, NY, United States.
• Santiago Sanchez-Vicente, Center for Infection and Immunity, Mailman School of Public Health, Columbia University, 722 West 168th Street, Room 1701, New York, NY 10032, United States.
• Gary P. Wormser, Division of Infectious Diseases, New York Medical College, Valhalla, NY, United States.Follow
• Luis A. Marcos, Department of Medicine (Division of Infectious Diseases), Department of Microbiology and Immunology, State University of New York at Stony Brook, Stony Brook, NY, United States.
• W Ian Lipkin, Center for Infection and Immunity, Mailman School of Public Health, Columbia University, 722 West 168th Street, Room 1701, New York, NY 10032, United States; Department of Epidemiology, Mailman School of Public Health, Columbia University, New York, NY, United States.
• Rafal Tokarz, Center for Infection and Immunity, Mailman School of Public Health, Columbia University, 722 West 168th Street, Room 1701, New York, NY 10032, United States; Department of Epidemiology, Mailman School of Public Health, Columbia University, New York, NY, United States. Electronic address: rt2249@cumc.columbia.edu.

Author Type(s)

Faculty

DOI

10.1016/j.ttbdis.2022.101999

Journal Title

Ticks and Tick-Borne Diseases

First Page

101999

Document Type

Article

Publication Date

9-1-2022

Department

Medicine

Keywords

Anaplasmosis, Babesiosis, Diagnosis, Microarray, Serology

Disciplines

Medicine and Health Sciences

Abstract

Anaplasma phagocytophilum and Babesia microti are emerging tick-borne pathogens in the United States. Although active infection is typically diagnosed by direct diagnostic tests, such as blood smear or polymerase chain reaction assay, serologic assays can be helpful to identify past infections, and the use of acute plus convalescent testing can potentially identify recent infections. We employed a peptide array to select sets of linear peptides for serologic diagnosis of infections with A. phagocytophilum and B. microti. Three optimal peptides were selected for each agent based on their performance with clinical specimens. All three A. phagocytophilum peptides were located within the conserved fragments of the MSP2 antigen. Two B. microti peptides were located in the N terminus of the SA-1 antigen; the third was in the BMN 1-17 antigen. We found that these peptides can be a useful tool for detection of antibody reactivity to both of these pathogens.

Recommended Citation

Tagliafierro, T., Joshi, S., Sameroff, S., Marques, A., Dumler, J. S., Mishra, N., Sanchez-Vicente, S., Wormser, G. P., Marcos, L. A., Lipkin, W. I., & Tokarz, R. (2022). Detection of Antibodies to Anaplasma Phagocytophilum and Babesia Microti Using Linear Peptides. Ticks and Tick-Borne Diseases, 13 (5), 101999. https://doi.org/10.1016/j.ttbdis.2022.101999