Upregulation of Exofacial Peroxiredoxin-Thioredoxin System of Lymphocytes and Monocytes in Preeclampsia

Authors

Bálint Alasztics, Department of Obstetrics and Gynecology, Semmelweis University, Budapest, Hungary; Department of Clinical Studies in Obstetrics and Gynecology, Faculty of Health Sciences, Semmelweis University, Budapest, Hungary.Follow
Árpád F. Kovács, 2(nd) Department of Pediatrics, Semmelweis University, Budapest, Hungary.
Éva Pállinger, Department of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, Hungary.
Katalin É. Szabó-Taylor, Department of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, Hungary.
Gábor Szabó, Department of Obstetrics and Gynecology, Semmelweis University, Budapest, Hungary.
Attila Molvarec, Department of Obstetrics and Gynecology, Semmelweis University, Budapest, Hungary.
Akos Koller, Department of Translational Medicine, Faculty of Medicine, Semmelweis University, Budapest, Hungary; Department of Morphology and Physiology, Faculty of Health Sciences, Semmelweis University, Budapest, Hungary; Department of Physiology, New York Medical College, Valhalla, NY 10595, USA.Follow
János Rigó, Department of Obstetrics and Gynecology, Semmelweis University, Budapest, Hungary; Department of Clinical Studies in Obstetrics and Gynecology, Faculty of Health Sciences, Semmelweis University, Budapest, Hungary. Electronic address: rigojanos31@gmail.com.

Author Type(s)

Faculty

DOI

10.1016/j.preghy.2022.12.002

Journal Title

Pregnancy Hypertension

First Page

54

Last Page

59

Document Type

Article

Publication Date

3-1-2023

Department

Physiology

Abstract

OBJECTIVES: An imbalanced redox homeostasis resulting in oxidative stress is present in preeclampsia. Peroxiredoxin-1 (PRDX1) and thioredoxin-1 (TRX1) regulatory enzymes are also contributing to the redox homeostasis, but were not investigated so far in preeclampsia. Thus, we have aimed to characterize PRDX1, TRX1 and oxidative stress biomarkers in blood samples of pregnant women with preeclampsia. STUDY DESIGN: Twelve patients with preeclampsia (PE) were enrolled into the study. Seven third trimester healthy pregnant women (HP) were accepted as control group. MAIN OUTCOME MEASURES: Peripheral venous blood samples of healthy and preeclamptic pregnant women were analyzed. Plasma level of advanced oxidation protein products (AOPP) was determined by spectrophotometry. The exofacial PRDX1 and TRX1 expression of lymphocytes and monocytes was detected by flow cytometry. RESULTS: The plasma AOPP level was significantly higher in preeclampsia compared to the healthy pregnant group. Significantly higher percentage of PRDX1 and TRX1 expressing lymphocytes and monocytes were detected in the blood samples of preeclamptic women compared to healthy pregnant controls. The ratio of circulating PRDX1 and TRX1 expressing lymphocytes and monocytes showed a significant inverse correlation with the birth weight of newborns. CONCLUSIONS: We have revealed that the level of advanced oxidation protein products is increased and the exofacial peroxiredoxin-1 and thioredoxin-1 system in lymphocytes and monocytes is upregulated in preeclampsia. In addition, the ratio of peroxiredoxin-1 and thioredoxin-1 positive circulating lymphocytes and monocytes correlates inversely with the neonatal birth weight, which finding indicates that pregnancies complicated by intrauterine growth restriction are accompanied by a higher level of oxidative stress.

Recommended Citation

Alasztics, B., Kovács, Á. F., Pállinger, É., Szabó-Taylor, K. É., Szabó, G., Molvarec, A., Koller, A., & Rigó, J. (2023). Upregulation of Exofacial Peroxiredoxin-Thioredoxin System of Lymphocytes and Monocytes in Preeclampsia. Pregnancy Hypertension, 31, 54-59. https://doi.org/10.1016/j.preghy.2022.12.002