Generation of Homogeneous Populations of Cortical Interneurons From Human Pluripotent Stem Cells

Authors

Peiyan Ni, Psychiatric Laboratory and Mental Health Center, The State Key Laboratory of Biotherapy, West China Hospital of Sichuan University, Chengdu, China.
Lingyi Fan, Psychiatric Laboratory and Mental Health Center, The State Key Laboratory of Biotherapy, West China Hospital of Sichuan University, Chengdu, China.
Amy Zinski, Department of Cell Biology and Anatomy, New York Medical College, Valhalla, NY, USA.
Sangmi Chung, Department of Cell Biology and Anatomy, New York Medical College, Valhalla, NY, USA. schung8@nymc.edu.

Author Type(s)

Faculty

Journal Title

Methods in Molecular Biology

First Page

13

Last Page

20

Document Type

Article

Publication Date

1-1-2023

Department

Cell Biology and Anatomy

Abstract

Cortical interneurons (cINs), especially those that are derived from the medial ganglionic eminence (MGE) during early development, are associated with various neuropsychiatric disorders. Human pluripotent stem cell (hPSC)-derived cINs can provide unlimited cell sources for studying disease mechanisms and developing novel therapeutics. Here, we describe an optimized method to generate homogeneous cIN populations based on three-dimensional (3D) cIN sphere generation. This optimized differentiation system could sustain generated cINs relatively long term without compromising their survival or phenotypes.

Recommended Citation

Ni, P., Fan, L., Zinski, A., & Chung, S. (2023). Generation of Homogeneous Populations of Cortical Interneurons From Human Pluripotent Stem Cells. Methods in Molecular Biology, 2683, 13-20. https://doi.org/10.1007/978-1-0716-3287-1_2