Generation of Homogeneous Populations of Cortical Interneurons From Human Pluripotent Stem Cells
Cell Biology and Anatomy
Cortical interneurons (cINs), especially those that are derived from the medial ganglionic eminence (MGE) during early development, are associated with various neuropsychiatric disorders. Human pluripotent stem cell (hPSC)-derived cINs can provide unlimited cell sources for studying disease mechanisms and developing novel therapeutics. Here, we describe an optimized method to generate homogeneous cIN populations based on three-dimensional (3D) cIN sphere generation. This optimized differentiation system could sustain generated cINs relatively long term without compromising their survival or phenotypes.
Ni, P., Fan, L., Zinski, A., & Chung, S. (2023). Generation of Homogeneous Populations of Cortical Interneurons From Human Pluripotent Stem Cells. https://doi.org/10.1007/978-1-0716-3287-1_2