NYMC Faculty Publications
N2 -substituted 2'-deoxyguanosine Triphosphate Derivatives as Selective Substrates for Human DNA Polymerase Kappa
Journal Title
Angewandte Chemie (International ed.in English)
First Page
2628
Last Page
2631
Document Type
Article
Publication Date
March 2017
Department
Biochemistry and Molecular Biology
Abstract
N2 -Alkyl-2'-deoxyguanosine triphosphate (N2 -alkyl-dGTP) derivatives with methyl, butyl, benzyl, or 4-ethynylbenzyl substituents were prepared and tested as substrates for human DNA polymerases. N2 -Benzyl-dGTP was equal to dGTP as a substrate for DNA polymerase kappa (pol kappa), but was a poor substrate for pols beta, delta, eta, iota, or nu. In vivo reactivity was evaluated through incubation of N2 -4-ethynylbenzyl-dG with wild-type and pol kappa deficient mouse embryonic fibroblasts. CuAAC reaction with 5(6)-FAM-azide demonstrated that only cells containing pol kappa were able to incorporate N2 -4-ethynylbenzyl-dG into the nucleus. This is the first instance of a Y-family-polymerase-specific dNTP, and this method could be used to probe the activity of pol kappa in vivo.
Recommended Citation
Gowda, A., Lee, M., & Spratt, T. (2017). N2 -substituted 2'-deoxyguanosine Triphosphate Derivatives as Selective Substrates for Human DNA Polymerase Kappa. Angewandte Chemie (International ed.in English), 56 (10), 2628-2631. https://doi.org/10.1002/anie.201611607
