NYMC Faculty Publications
Aging-induced Modulation of Pituitary Adenylate Cyclase-activating Peptide- and Vasoactive Intestinal Peptide-induced Vasomotor Responses in the Arteries of Mice
DOI
10.1159/000481781
Journal Title
Journal of Vascular Research
First Page
359
Last Page
366
Document Type
Article
Publication Date
January 2017
Department
Physiology
Abstract
Pituitary adenylate cyclase-activating peptide (PACAP; 1-38 and 1-27) and vasoactive intestinal peptide (VIP) are related neuropeptides of the secretin/glucagon family. Overlapping signaling through G-protein-coupled receptors mediates their vasomotor activity. We previously showed that PACAP deficiency (PACAP-KO) shifts the mechanisms of vascular response and maintains arterial relaxation through the VIP backup mechanism and (mainly) its VPAC1R, but their agedependent modulation is still unknown. We hypothesized that backup mechanisms exist, which maintain the vasomotor activity of these peptides also in older age. Thus, we investigated the effects of exogenous VIP and PACAP peptides in isolated carotid arteries of 2-and 15-month-old wild-type (WT) and PACAP-KO mice. All peptides induced relaxation in the arteries of young WT mice, whereas in young PACAP-KO mice PACAP1-27 and VIP, but not PACAP1-38, induced relaxation. Unlike VIP, PACAP-induced vasomotor responses were reduced in aging WT mice. However, in the arteries of aging PACAP-KO mice, PACAP1-27-and VIP-induced responses were reduced, but PACAP1-38 showed a greater vasomotor response compared to that of young PACAP-KO animals. There were no significant differences between the vasomotor responses of aging WT and PACAP-KO mice. Our data suggest that, in the absence of PACAP both in young and old ages, the vascular response is mediated through backup mechanisms, most likely VIP, maintaining proper vascular relaxation in aging-induced PACAP insufficiency. (c) 2017 S. Karger AG, Basel
Recommended Citation
Ivic, I., Solymar, M., Fulop, B., Hashimoto, H., Toth, G., Tamas, A., Juhasz, T., Koller, A., & Reglodi, D. (2017). Aging-induced Modulation of Pituitary Adenylate Cyclase-activating Peptide- and Vasoactive Intestinal Peptide-induced Vasomotor Responses in the Arteries of Mice. Journal of Vascular Research, 54 (6), 359-366. https://doi.org/10.1159/000481781