NYMC Faculty Publications
Long-Term Efficacy of Liposomal Nanocarriers of Preassembled Glycocalyx in Restoring Cerebral Endothelial Glycocalyx in Sepsis
Author Type(s)
Faculty
DOI
10.1016/j.mvr.2024.104684
Journal Title
Microvascular Research
First Page
104684
Document Type
Article
Publication Date
7-1-2024
Department
Physiology
Abstract
The endothelial glycocalyx (EG) undergoes early degradation in sepsis. Our recent work introduced a novel therapeutic approach involving liposomal nanocarriers of preassembled glycocalyx (LNPG) to restore EG in lipopolysaccharide (LPS)-induced sepsis model of mice. While short-term effects were promising, this study focuses on the long-term impact of LNPG on mouse cerebral microcirculation. Utilizing cranial window, we assessed the stability of vascular density (VD) and perfused boundary region (PBR), an index of EG thickness, over a five-day period in normal control mice. In septic groups (LPS, LPS + 1-dose LNPG, and LPS + 2-dose LNPG), the exposure of mice to LPS significantly reduced VD and increased PBR within 3 h. Without LNPG treatment, PBR returned to the normal control level by endogenous processes at 48 h, associated with the recovery of VD to the baseline level at 72 h. However, mice receiving LNPG treatment significantly reduced the increment of PBR at 3 h. The therapeutic effect of 1-dose LNPG persisted for 6 h while the 2-dose LNPG treatment further reduced PBR and significantly increased VD at 12 h compared to LPS group. This study provides valuable insights into the potential therapeutic benefits of LNPG in mitigating EG degradation in sepsis.
Recommended Citation
Ishiko, S., Huang, A., & Sun, D. (2024). Long-Term Efficacy of Liposomal Nanocarriers of Preassembled Glycocalyx in Restoring Cerebral Endothelial Glycocalyx in Sepsis. Microvascular Research, 154, 104684. https://doi.org/10.1016/j.mvr.2024.104684