NYMC Faculty Publications
HPLC Determination of Alpha-Ketoglutaramate [5-Amino-2,5-Dioxopentanoate] in Biological Samples
First Page
52
Last Page
54
Document Type
Article
Publication Date
2-1-2016
Department
Biochemistry and Molecular Biology
Abstract
α-Ketoglutaramate is an important glutamine metabolite in mammals, plants, and many bacteria. It is also a nicotine metabolite in certain bacteria. Previously published methods for the determination of α-ketoglutaramate in biological samples have considerable drawbacks. Here, we describe a relatively simple high-performance liquid chromatography (HPLC)-based method for measurement of α-ketoglutaramate in plasma and deproteinized tissues that overcomes these drawbacks. Concentrations of α-ketoglutaramate in normal rat liver, kidney, brain, and plasma were found to be approximately 216, 13, 6, and 19 μM, respectively. The HPLC method should be useful for studying the role of α-ketoglutaramate in eukaryotic glutamine metabolism and in bacterial nicotine metabolism.
Recommended Citation
Shurubor, Y. I., Cooper, A. J., Isakova, E. P., Deryabina, Y. I., Beal, M. F., & Krasnikov, B. F. (2016). HPLC determination of alpha-ketoglutaramate [5-amino-2,5-dioxopentanoate] in biological samples. Analytical Biochemistry, 494, 52-54. doi:10.1016/j.ab.2015.11.003
Publisher's Statement
Originally published in Analytical Biochemistry. https://doi.org/10.1016/j.ab.2015.11.003