NYMC Faculty Publications
Modification of Expanded NK Cells With Chimeric Antigen Receptor Mrna for Adoptive Cellular Therapy
Author Type(s)
Faculty
DOI
10.1007/978-1-4939-3684-7_18
Journal Title
Methods in Molecular Biology
First Page
215
Last Page
30
Document Type
Article
Publication Date
1-1-2016
Abstract
NK cells are bone marrow-derived cytotoxic lymphocytes that play a major role in the rejection of tumors and cells infected by viruses. The regulation of NK activation vs inhibition is regulated by the expression of a variety of NK receptors (NKRs) and specific NKRs' ligands expressed on their targets. However, factors limiting NK therapy include small numbers of active NK cells in unexpanded peripheral blood and lack of specific tumor targeting. Chimeric antigen receptors (CAR) usually include a single-chain Fv variable fragment from a monoclonal antibody, a transmembrane hinge region, and a signaling domain such as CD28, CD3-zeta, 4-1BB (CD137), or 2B4 (CD244) endodimers. Redirecting NK cells with a CAR will circumvent the limitations of the lack of NK targeting specificity. This chapter focuses on the methods to expand human NK cells from peripheral blood by co-culturing with feeder cells and to modify the expanded NK cells efficiently with the in vitro transcribed CAR mRNA by electroporation and to test the functionality of the CAR-modified expanded NK cells for use in adoptive cellular immunotherapy.
Recommended Citation
Chu, Y., Flower, A., & Cairo, M. S. (2016). Modification of Expanded NK Cells With Chimeric Antigen Receptor Mrna for Adoptive Cellular Therapy. Methods in Molecular Biology, 1441, 215-30. https://doi.org/10.1007/978-1-4939-3684-7_18