NYMC Faculty Publications
Age Determines the Magnitudes of Angiotensin II-Induced Contractions, Mrna, and Protein Expression of Angiotensin Type 1 Receptors in Rat Carotid Arteries
Author Type(s)
Faculty
DOI
10.1093/gerona/glt128
Journal Title
The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences
First Page
519
Last Page
26
Document Type
Article
Publication Date
5-1-2014
Abstract
In this study, we hypothesized that aging alters angiotensin II (Ang II)-induced vasomotor responses and expression of vascular mRNA and protein angiotensin type 1 receptor (AT1R). Thus, carotid arteries were isolated from the following age groups of rats: 8 days, 2-9 months, 12-20 months, and 20-30 months, and their vasomotor responses were measured in a myograph after repeated administrations of Ang II. Vascular relative AT1R mRNA level was determined by quantitative reverse-transcriptase polymerase chain reaction and the AT1R protein density was measured by Western blot. Contractions to the first administration of Ang II increased from 8 days to 6 months and then they decreased to 30 months. In general, second administration of Ang II elicited reduced contractions, but they also increased from 8 days until 2 months and then they decreased to 30 months. Similarly the AT1R mRNA level increased from 8 days to 12 months and then decreased to 30 months. Similarly the AT1R protein density increased from 8 days until 16 months and then they decreased to 30 months. The pattern of these changes correlated with functional vasomotor data. We conclude that aging (newborn to senescence) has substantial effects on Ang II-induced vasomotor responses and AT1R signaling suggesting the importance of genetic programs.
Recommended Citation
Vamos, Z., Cseplo, P., Ivic, I., Matics, R., Hamar, J., & Koller, A. (2014). Age Determines the Magnitudes of Angiotensin II-Induced Contractions, Mrna, and Protein Expression of Angiotensin Type 1 Receptors in Rat Carotid Arteries. The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences, 69 (5), 519-26. https://doi.org/10.1093/gerona/glt128