NYMC Faculty Publications
Measurement of Sulfur-Containing Compounds Involved in the Metabolism and Transport of Cysteamine and Cystamine. Regional Differences in Cerebral Metabolism
First Page
3434
Last Page
3441
Document Type
Article
Publication Date
10-15-2009
Department
Biochemistry and Molecular Biology
Keywords
Animals, Biological Transport, Cerebrum, Chromatography, High Pressure Liquid, Cystamine, Cysteamine, Male, Oxidation-Reduction, Rats, Rats, Sprague-Dawley, Sulfur Compounds
Disciplines
Biochemistry | Molecular Biology
Abstract
An HPLC method with coulometric detection is presented for the quantitation of cysteamine, cystamine, thialysine, glutathione, glutathione disulfide and an oxidized metabolite of thialysine [S-(2-aminoethyl)-L-cysteine ketimine decarboxylated dimer (AECK-DD)]. The advantage of coulometric detection is that derivatization is unnecessary if the analyte is redox sensitive. The method was used to quantitate several sulfur-containing compounds in plasma and brain following gavage feeding of cysteamine to rats. Cysteamine, cystamine, thialysine and AECK-DD were detected in the brains of these animals. Interestingly, cysteamine treatment resulted in greatly elevated levels of cerebral methionine, despite the fact that cysteamine is not a precursor of methionine.
Recommended Citation
Pinto, J. T., Khomenko, T., Szabo, S., McLaren, G. D., Denton, T. T., Krasnikov, B. F., et al. (2009). Measurement of sulfur-containing compounds involved in the metabolism and transport of cysteamine and cystamine. regional differences in cerebral metabolism. Journal of Chromatography.B, Analytical Technologies in the Biomedical and Life Sciences, 877(28), 3434-3441. doi:10.1016/j.jchromb.2009.05.041
Publisher's Statement
Originally published in Journal of Chromatography.B, Analytical Technologies in the Biomedical and Life Sciences. https://doi.org/10.1016/j.jchromb.2009.05.041
Comments
Please see the work itself for the complete list of authors.