NYMC Faculty Publications

Rat Incisor Dentine Contains a Factor Which Alters the Phenotypic Expression and Stimulates Chondrogenesis in Fibroblast-Like Cells in Vitro

Author Type(s)

Faculty

Additional Author Affiliation

Touro College of Dental Medicine at NYMC

Journal Title

Biomaterials

First Page

35

Last Page

37

Document Type

Article

Publication Date

7-1-1990

Department

Pharmacology

Abstract

A low molecular weight protein fraction isolated under dissociative conditions during the demineralization of rat incisor dentine has the ability to modulate, in culture, the expression of fibroblast-like cells explanted from neonatal rat muscle. The protein fraction enhances the incorporation of 35S-sulphate into a proteoglycan larger in weight than that produced by the uninduced cells; furthermore it induces the production of type II collagen. These changes take place in the absence of cell proliferation as measured by 3H-thymidine incorporation. The altered fibroblast-like cells form nodules and secrete an abundant extracellular matrix which stains for proteoglycan after 7-9 days in culture. These data show that the dentine matrix does contain a factor which can initiate a mitogenesis-independent alteration in the expression of the muscle-explant outgrowth cells. Those changes are consistent with a shift to a chondrogenic mode.

Link to Full Text

Share

COinS