NYMC Faculty Publications
LITAF Mediation of Increased TNF-α Secretion From Inflamed Colonic Lamina Propria Macrophages
Author Type(s)
Faculty
Additional Author Affiliation
Touro College of Dental Medicine at NYMC
DOI
10.1371/journal.pone.0025849
Journal Title
PLoS One
First Page
25849
Last Page
25849
Document Type
Article
Publication Date
1-1-2011
Department
Pharmacology
Keywords
Animals, Blotting, Western, Colon, DNA-Binding Proteins, Enzyme-Linked Immunosorbent Assay, Inflammation, Lipopolysaccharides, Macrophages, Mice, Mice, Inbred C57BL, Mucous Membrane, Nuclear Proteins, Peroxidase, Reverse Transcriptase Polymerase Chain Reaction, Transcription Factors, Tumor Necrosis Factor-alpha
Disciplines
Medicine and Health Sciences
Abstract
Dysregulation of TNF-α in lamina propria macrophages (LPM) is a feature of inflammatory bowel diseases (IBD). LPS-Induced-TNF-Alpha-Factor (LITAF) is a transcription factor that mediates TNF-α expression. To determine whether LITAF participates in the mediation of TNF-α expression in acutely inflamed colonic tissues, we first established the TNBS-induced colonic inflammation model in C57BL/6 mice. LPM were harvested from non-inflamed and inflamed colonic tissue and inflammatory parameters TNF-α and LITAF mRNA and protein levels were measured ex-vivo. LPM from TNBS-treated mice secreted significantly more TNF-α at basal state and in response to LPS than LPM from untreated mice (p
Recommended Citation
Bushell, K. N., Leeman, S. E., Gillespie, E., Gower, A. C., Reed, K. L., Stucchi, A. F., Becker, J. M., & Amar, S. (2011). LITAF Mediation of Increased TNF-α Secretion From Inflamed Colonic Lamina Propria Macrophages. PLoS One, 6 (9), 25849-25849. https://doi.org/10.1371/journal.pone.0025849
