NYMC Faculty Publications
A Monoclonal Antibody Targeting a Highly Conserved Epitope in Influenza B Neuraminidase Provides Protection Against Drug Resistant Strains
Author Type(s)
Faculty
Journal Title
Biochemical and Biophysical Research Communications
First Page
226
Last Page
229
Document Type
Article
Publication Date
11-8-2013
Abstract
All influenza viral neuraminidases (NA) of both type A and B viruses have only one universally conserved sequence located between amino acids 222-230. A monoclonal antibody against this region has been previously reported to provide broad inhibition against all nine subtypes of influenza A NA; yet its inhibitory effect against influenza B viral NA remained unknown. Here, we report that the monoclonal antibody provides a broad inhibition against various strains of influenza B viruses of both Victoria and Yamagata genetic lineage. Moreover, the growth and NA enzymatic activity of two drug resistant influenza B strains (E117D and D197E) are also inhibited by the antibody even though these two mutations are conformationally proximal to the universal epitope. Collectively, these data suggest that this unique, highly-conserved linear sequence in viral NA is exposed sufficiently to allow access by inhibitory antibody during the course of infection; it could represent a potential target for antiviral agents and vaccine-induced immune responses against diverse strains of type B influenza virus.
Recommended Citation
Doyle, T., Li, C., Bucher, D., Hashem, A., Van Domselaar, G., Wang, J., Farnsworth, A., She, Y., Cyr, T., He, R., Brown, E., Hurt, A., & Li, X. (2013). A Monoclonal Antibody Targeting a Highly Conserved Epitope in Influenza B Neuraminidase Provides Protection Against Drug Resistant Strains. Biochemical and Biophysical Research Communications, 441 (1), 226-229. Retrieved from https://touroscholar.touro.edu/nymc_fac_pubs/5818
