Harmonizing Lipidomics: NIST Interlaboratory Comparison Exercise for Lipidomics Using SRM 1950-metabolites in Frozen Human Plasma

Authors

J Bowden
C Ulmer
C Jones
J Koelmel
L Abdullah
Houli Jiang, New York Medical CollegeFollow
Michal Schwartzman, New York Medical CollegeFollow
Amaury Cazenave-Gassiot
Antonio Checa
Michelle Cinel
Romain Colas
Serge Cremers
Edward Dennis
James Evans
Alexander Fauland
Jun Han
Houli Jiang, New York Medical CollegeFollow
Michal Schwartzman, New York Medical CollegeFollow

DOI

10.1194/jlr.M079012

Journal Title

Journal of Lipid Research

First Page

2275

Last Page

2288

Document Type

Article

Publication Date

12-1-2017

Department

Pharmacology

Abstract

As the lipidomics field continues to advance, self-evaluation within the community is critical. Here, we performed an interlaboratory comparison exercise for lipidomics using Standard Reference Material (SRM) 1950-Metabolites in Frozen Human Plasma, a commercially available reference material. The interlaboratory study comprised 31 diverse laboratories, with each laboratory using a different lipidomics workflow. A total of 1,527 unique lipids were measured across all laboratories and consensus location estimates and associated uncertainties were determined for 339 of these lipids measured at the sum composition level by five or more participating laboratories. These evaluated lipids detected in SRM 1950 serve as community-wide benchmarks for intra-and interlaboratory quality control and method validation. These analyses were performed using nonstandardized laboratory-independent workflows. The consensus locations were also compared with a previous examination of SRM 1950 by the LIPID MAPS consortium.jlr While the central theme of the interlaboratory study was to provide values to help harmonize lipids, lipid mediators, and precursor measurements across the community, it was also initiated to stimulate a discussion regarding areas in need of improvement.

Comments

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Recommended Citation

Bowden, J., Ulmer, C., Jones, C., Koelmel, J., Abdullah, L., Jiang, H., Schwartzman, M., Cazenave-Gassiot, A., Checa, A., Cinel, M., Colas, R., Cremers, S., Dennis, E., Evans, J., Fauland, A., Han, J., Jiang, H., & Schwartzman, M. (2017). Harmonizing Lipidomics: NIST Interlaboratory Comparison Exercise for Lipidomics Using SRM 1950-metabolites in Frozen Human Plasma. Journal of Lipid Research, 58 (12), 2275-2288. https://doi.org/10.1194/jlr.M079012

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