NYMC Faculty Publications

Stem Cells of the Lamina Propria of Human Oral Mucosa and Gingiva Develop into Mineralized Tissues in Vivo

Author Type(s)

Faculty

Additional Author Affiliation

Touro College of Dental Medicine at NYMC

DOI

10.1111/jcpe.12016

Journal Title

Journal of Clinical Periodontology

First Page

73

Last Page

81

Document Type

Article

Publication Date

1-1-2013

Department

Pathology, Microbiology and Immunology

Keywords

Alkaline Phosphatase, Amelogenin, Animals, Bone Regeneration, Collagen, Dental Cementum, Fibrin, Gingiva, Humans, Integrin-Binding Sialoprotein, Mice, Mice, SCID, Mouth Mucosa, Osteocalcin, Protein Tyrosine Phosphatases, Proteins, Regeneration, Stem Cell Transplantation, Stem Cells

Disciplines

Medicine and Health Sciences

Abstract

AIMS: To characterize the mineralized tissue formed constitutively in the supracalvarial region of scid mice by a primitive stem cell population (hOMSC) derived from the lamina propria of the human oral mucosa and gingiva.

MATERIAL AND METHODS: Fibrin-hOMSC constructs were cultured for 14 days at which time point they were analysed for the expression of osteoblastic/cementoblastic markers and implanted between the skin and calvaria bones into scid mice. After 8 weeks, the animals were sacrificed and the implantation sites analysed.

RESULTS: Two-week-old cultures of fibrin-hOMSC constructs expressed osteogenic/cementogenic markers at the gene level. Macroscopic and radiographic examinations revealed mineralized masses at the implantation sites of fibrin-hOMSC constructs. Histology, histochemistry and immunofluorescence showed mineralized masses consisting of avascular cellular and acellular matrices that stained positively for collagen, Ca, cementum attachment protein, cementum protein 1, bone sialoprotein, alkaline phosphatase, osteocalcin, amelogenin and ameloblastin. Positive anti-human nuclear antigen indicated the human origin of the cells. Atomic force microscopy depicted long prismatic structures organized in lamellar aggregates.

CONCLUSIONS: Within the limitation of this study, the results indicate for the first time that fibrin-hOMSC constructs are endowed with the constitutive capacity to develop into mineralized tissues that exhibit certain similarities to cementum and bone.

Link to Full Text

Share

COinS