Hypoacetylation, Hypomethylation, and Dephosphorylation of H2B Histones and Excessive Histone Deacetylase Activity in DU-145 Prostate Cancer Cells

Authors

Shundong Cang
Xiaobin Xu
Yuehua Ma
Delong Liu, New York Medical CollegeFollow
JenWei Chiao, New York Medical CollegeFollow

First Page

3

Last Page

3

Document Type

Article

Publication Date

1-12-2016

Department

Medicine

Abstract

BACKGROUND: Hypoacetylation on histone H3 of human prostate cancer cells has been described. Little is known about the modifications of other histones from prostate cancer cells.

METHODS: Histones were isolated from the prostate cancer cell line DU-145 and the non-malignant prostatic cell line RC170N/h. Post-translational modifications of histone H2B were determined by liquid chromatography-mass spectrometry (LC-MS)/MS.

RESULTS: The histone H2B of the prostate cancer cell line DU-145 was found to have hypoacetylation, hypomethylation, and dephosphorylation as compared to the non-malignant prostatic cell line RC170N/h. H2B regained acetylation on multiple lysine residues, phosphorylation on Thr19, and methylation on Lys23 and Lys43 in the DU-145 cells after sodium butyrate treatment.

CONCLUSIONS: The histone H2B of DU-145 prostate cancer cells are hypoacetylated, hypomethylated, and dephosphorylated. Histone deacetylase inhibitor reversed this phenotype. Epigenetic agent may therefore be useful for prostate cancer therapy and worth further investigation.

Recommended Citation

Cang, S., Xu, X., Ma, Y., Liu, D., & Chiao, J. W. (2016). Hypoacetylation, hypomethylation, and dephosphorylation of H2B histones and excessive histone deacetylase activity in DU-145 prostate cancer cells. Journal of Hematology & Oncology, 9, 3. doi:10.1186/s13045-016-0233-x

Publisher's Statement

Originally published in Journal of Hematology & Oncology. Licensed under CC-BY 4.0. https://doi.org/10.1186/s13045-016-0233-x