NYMC Faculty Publications
Determination of Coenzyme A and Acetyl-Coenzyme A in Biological samples Using HPLC with UV Detection
DOI
10.3390/molecules22091388
Journal Title
Molecules (Basel, Switzerland)
First Page
1388
Document Type
Article
Publication Date
8-1-2017
Department
Biochemistry and Molecular Biology
Keywords
Acetyl Coenzyme A, Chromatography, High Pressure Liquid, Coenzyme A, Spectrophotometry, Ultraviolet, UV detection;
Disciplines
Biochemistry | Medicine and Health Sciences | Molecular Biology
Abstract
Coenzyme A (CoA) and acetyl-coenzyme A (acetyl-CoA) play essential roles in cell energy metabolism. Dysregulation of the biosynthesis and functioning of both compounds may contribute to various pathological conditions. We describe here a simple and sensitive HPLC-UV based method for simultaneous determination of CoA and acetyl-CoA in a variety of biological samples, including cells in culture, mouse cortex, and rat plasma, liver, kidney, and brain tissues. The limits of detection for CoA and acetyl-CoA are >10-fold lower than those obtained by previously described HPLC procedures, with coefficients of variation
Recommended Citation
Shurubor, Y., D'Aurelio, M., Clark-Matott, J., Isakova, E., Deryabina, Y., Beal, M., Cooper, A., & Krasnikov, B. (2017). Determination of Coenzyme A and Acetyl-Coenzyme A in Biological samples Using HPLC with UV Detection. Molecules (Basel, Switzerland), 22 (9), 1388. https://doi.org/10.3390/molecules22091388
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
