Biochemistry and Molecular Biology
Coenzyme A (CoA) and acetyl-coenzyme A (acetyl-CoA) play essential roles in cell energy metabolism. Dysregulation of the biosynthesis and functioning of both compounds may contribute to various pathological conditions. We describe here a simple and sensitive HPLC-UV based method for simultaneous determination of CoA and acetyl-CoA in a variety of biological samples, including cells in culture, mouse cortex, and rat plasma, liver, kidney, and brain tissues. The limits of detection for CoA and acetyl-CoA are >10-fold lower than those obtained by previously described HPLC procedures, with coefficients of variation
Shurubor, Y., D'Aurelio, M., Clark-Matott, J., Isakova, E., Deryabina, Y., Beal, M., Cooper, A., & Krasnikov, B. (2017). Determination of Coenzyme A and Acetyl-Coenzyme A in Biological samples Using HPLC with UV Detection. Molecules (Basel, Switzerland), 22 (9), 1388. https://doi.org/10.3390/molecules22091388
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