Document Type
Article
Publication Date
2016
Abstract
Measurements of glycolytic rate and maximum glycolytic capacity using extracellular flux analysis can give crucial information about cell status and phenotype during normal operation, development of pathology, differentiation, and malignant transformation. They are also of great use when assessing the effects of chemical or drug treatments. Here, we experimentally define maximum glycolytic capacity, demonstrate how it differs from glycolytic rate, and provide a protocol for determining the basal glycolytic rate and maximum glycolytic capacity in cells using extracellular flux measurements. The results illustrate the power of extracellular flux analysis to describe the energetics of adherent cells in culture in a fully quantitative way.
Recommended Citation
Mookerjee, S. A., Nicholls, D. G., & Brand, M. D. (2016). Determining maximum glycolytic capacity using extracellular flux measurements. PLOS One, 11(3) [Article e0152016].
Publisher's Statement
Originally published in PLOS One, 11(3) [Article e0152016]. Licensed under CC BY 4.0. doi:10.1371/journal.pone.0152016
