Measurement of Sulfur-Containing Compounds Involved in the Metabolism and Transport of Cysteamine and Cystamine. Regional Differences in Cerebral Metabolism

Authors

John T. Pinto, New York Medical CollegeFollow
Gordon McLaren
Travis Denton
Boris F. Krasnikov, New York Medical CollegeFollow
Thomas M. Jeitner, New York Medical CollegeFollow
Arthur J L Cooper, New York Medical CollegeFollow

First Page

3434

Last Page

3441

Document Type

Article

Publication Date

10-15-2009

Department

Biochemistry and Molecular Biology

Abstract

An HPLC method with coulometric detection is presented for the quantitation of cysteamine, cystamine, thialysine, glutathione, glutathione disulfide and an oxidized metabolite of thialysine [S-(2-aminoethyl)-L-cysteine ketimine decarboxylated dimer (AECK-DD)]. The advantage of coulometric detection is that derivatization is unnecessary if the analyte is redox sensitive. The method was used to quantitate several sulfur-containing compounds in plasma and brain following gavage feeding of cysteamine to rats. Cysteamine, cystamine, thialysine and AECK-DD were detected in the brains of these animals. Interestingly, cysteamine treatment resulted in greatly elevated levels of cerebral methionine, despite the fact that cysteamine is not a precursor of methionine.

Comments

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Recommended Citation

Pinto, J. T., Khomenko, T., Szabo, S., McLaren, G. D., Denton, T. T., Krasnikov, B. F., et al. (2009). Measurement of sulfur-containing compounds involved in the metabolism and transport of cysteamine and cystamine. regional differences in cerebral metabolism. Journal of Chromatography.B, Analytical Technologies in the Biomedical and Life Sciences, 877(28), 3434-3441. doi:10.1016/j.jchromb.2009.05.041

Publisher's Statement

Originally published in Journal of Chromatography.B, Analytical Technologies in the Biomedical and Life Sciences. https://doi.org/10.1016/j.jchromb.2009.05.041