NYMC Faculty Publications
Mechanism of Formation of Non-immune Rosettes Between Guinea-Pig Thymus-Derived Lymphocytes and Rabbit Erythrocytes
Journal Title
Immunology
First Page
25
Last Page
31
Document Type
Article
Publication Date
January 1977
Department
Pathology, Microbiology and Immunology
Abstract
Several observations reported here suggest that spontaneous rosette formation between rabbit erythrocytes and guinea-pig T lymphocytes is mediated by natural anti-guinea-pig T-cell antibodies bound to the surface of the rabbit erythrocyte. First, normal rabbit sera frequently contain antibodies specifically cytotoxic for guinea-pig T lymphocytes. Second, the activity of rabbit erythrocytes in spontaneous rosette formation is reduced after incubation for 5 days at pH 6-1, but can be restored to levels seen with fresh erythrocytes by a brief incubation in normal rabbit serum containing natural anti-guinea-pig antibodies; normal serum absorbed with thymocytes does not restore activity to the erythrocytes. Third, the activity of rabbit erythrocytes in forming spontaneous rosettes can be specifically blocked by treatment with anti-allotype and heterologous anti-Ig sera.
Recommended Citation
Godfrey, H. P. (1977). Mechanism of Formation of Non-immune Rosettes Between Guinea-Pig Thymus-Derived Lymphocytes and Rabbit Erythrocytes. Immunology, 32 (1), 25-31. Retrieved from https://touroscholar.touro.edu/nymc_fac_pubs/1702