NYMC Faculty Publications

Differences in Sensitivity to a Cytotoxic Anti-Thymus-Derived Lymphocyte Serum of Cells Mediating Delayed-Onset Reactions in Guinea Pigs to Hapten-Protein Conjugates and Contactants

DOI

10.1016/0008-8749(76)90004-6

Journal Title

Cellular Immunology

First Page

28

Last Page

42

Document Type

Article

Publication Date

March 1976

Department

Pathology, Microbiology and Immunology

Abstract

Lymph node cells from guinea pigs immunized with a reactive dinitrophenyl (DNP) compound in complete Freund's adjuvant were treated with a cytotoxic anti-guinea pig thymus-derived lymphocyte (T cell) antiserum prior to transfer to unimmunized recipients to study the functional cell types active in mediating delayed-onset hypersensitivities. Attempts were also made to block the passive transfer of delayed-onset sensitivities with an anti-guinea pig κ chain serum. The data indicate that delaye-donset sensitivities to contactant and to PPD are mediated by T cells easily killed by a high dilution of anti-T cell serum but not affected by a low dilution of anti-κ chain serum. Surprisingly, the delayed-onset response to DNP conjugates was undiminished after treatment with anti-T cell serum which suggests that this sensitivity is not mediated by cells mediating the other delayed-onset sensitivities. In both actively and passively sensitized animals, contact sensitivity was highly specific for DNP; in contrast, delayed-onset sensitivity to conjugates was elicited nearly as well by DNP as by TNP conjugates, a characteristic cross-reactivity often seen in serum antibodies to DNP. Despite the differences among them, all three types of delaye-donset sensitivity were cell-mediated and could not be passively transferred by heat-killed cells or serum.

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