NYMC Faculty Publications
PP128. Placental Caspase-3 Gene Polymorphisms is Associated With Preeclampsia
Author Type(s)
Faculty
DOI
10.1016/j.preghy.2012.04.239
Journal Title
Pregnancy Hypertension
First Page
308
Last Page
308
Document Type
Article
Publication Date
7-1-2012
Department
Pediatrics
Disciplines
Medicine and Health Sciences
Abstract
INTRODUCTION: Increased placental trophoblastic apoptosis (programmed cell death) was previously reported in pregnancies complicated by preeclampsia. Caspase-3 is one of the key executioners of apoptosis. Caspase are expressed in many tissues including human placental trophoblast and other tissues. Variations in the promoter area of the Caspase genes may modulate apoptotic signaling, contributing to an increased risk of preeclampsia
OBJECTIVES: To determine if gene polymorphisms of Caspase 3 proteins differ between patient with and without preeclampsia.
METHODS: Forty-three singleton placentas were studied. Twenty-two placentas were with preeclampsia and 21 were normotensive controls. DNA was extracted from placentas using QIAAmp DNA Minikit. Genotyping of Caspase 3 +567 was determined by real-time PCR using the Applied Biosystems Prism 7900 HT SDS machine. Chi-square and Fisher's exact tests were used for statistical analysis.
RESULTS: There were no significant differences in maternal age, parity or race between the two groups. Preeclamptic placentas had higher frequency of wild type TT of Caspase-3 SNP (+567) as compared with normotensive controls (59% versus 28.5%). Preeclamptic placentas expressed significantly more genotype of TT of Caspase-3 SNP (+567) than normotensive patients when compared to CC (p=0.02). The alle frequencies of the Caspase SNP (+567) in preeclampstic placentas were 0.77 and 0.23 for T and C, respectively, as compared to 0.52 and 0.48, respectively, in placentas from normotensive pregnancies.
CONCLUSION: Immune intolerance of maternal and placental interaction plays an important role in the pathogenesis of preeclampsia. Increased of placental apoptosis was reported in pregnancy complicated with preeclamsia. Our findings indicate placental Caspase 3 (+567) gene polymorphisms is associated with preeclampsia. Altered placental alle frequencies and caspase-3 SNP (+567) in preeclampsia further suggests preeclampsia is a trophoblastic disorder.
Recommended Citation
Hsu, C., Polavarapu, S., & Parton, L. A. (2012). PP128. Placental Caspase-3 Gene Polymorphisms is Associated With Preeclampsia. Pregnancy Hypertension, 2 (3), 308-308. https://doi.org/10.1016/j.preghy.2012.04.239
